HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1. Positive lysates LY414526 (100ug) and LC414526 (20ug) can be purchased separately from OriGene.
Anti-SAMHD1 mouse monoclonal antibody (TA501954) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1 (RC206013).
Western blot analysis of extracts (10ug) from 8 different cell lines by using anti-SAMHD1 monoclonal antibody at 1:200 dilution.
Figure from citation: Western Blot of SAMHD1 protein level by using anti-SAMHD1 antibody in lysates from parental U937 or U937 expressing human or mouse SAMHD1. Dilution: 1:1000 View Citation
Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody (TA501954), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-SAMHD1 antibody (TA501954), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).