Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SAMHD1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Anti-SAMHD1 mouse monoclonal antibody (TA502085) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SAMHD1 (RC206013).
Flow cytometric Analysis of Hela cells, using anti-SAMHD1 antibody (TA502085), (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SAMHD1 (RC206013, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SAMHD1. Positive lysates LY414526 (100ug) and LC414526 (20ug) can be purchased separately from OriGene.