Flow cytometric Analysis of Hela cells, using anti-TRIM2 antibody (TA501606), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM2 (RC229368, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM2. Positive lysates LY414662 (100ug) and LC414662 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC203947 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TRIM2 antibody (TA501606), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-TRIM2 antibody (TA501606), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TRIM2 monoclonal antibody.