HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MGRN1 (RC208284, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MGRN1. Positive lysates LY414714 (100ug) and LC414714 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-MGRN1 monoclonal antibody.
Flow cytometric Analysis of Jurkat cells, using anti-MGRN1 antibody (TA502681), (Red), compared to a nonspecific negative control antibody, (Blue).
Anti-MGRN1 mouse monoclonal antibody (TA502681) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY MGRN1 (RC208284).
HEK293T cells transfected with either RC208284 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MGRN1 antibody (TA502681), and then analyzed by flow cytometry.