RNA STAT-60
Three-in-one: RNA, DNA, and protein extraction reagent
Recent progress in RNA isolation technology has made it possible to replace lengthy and laborious methods of total RNA isolation by a single-step method.
RNA STAT-60 is a ready-to-use reagent that can be used either for RNA, DNA or protein extraction from tissues or cells of human, animal, plant, bacterial and viral origin. Extensive laboratory tests have shown that the RNA STAT-60 is highly reliable and produces consistent results. The simplicity of isolation using the RNA STAT-60 enables the efficient recovery of high quality RNA from very small biological samples, making it possible to simultaneously process a large number of samples. The total RNA isolated by the RNA STAT-60 is undegraded and free of protein and DNA contamination.
Features
- Isolates total RNA/mRNA in under 60 minutes
- Northern blot/PCR - ready mRNA obtained in under 60 minutes
- No further purification required for use in subsequent procedures including Northern blotting and PCR
- Extracts up to 150% more total RNA/mRNA than any other method
- Cost effective method requiring less reagent & sample
- Has been used for gene expression analysis in organoid culture (see Knouse et al., 2018, Cell 175, 200–211)
- Additional protocols to isolate DNA and Protein
Applications
- Northern blot, and PCR without additional treatment with DNase
- Dot blot hybridization
- Poly A+ selection
- Molecular cloning
- In vitro translation
- RNase protection assay
Citations
Chromosome segregation fidelity in epithelia requires tissue architecture
Knouse, K. A. et al. (2018) Cell. 175: 200-211