Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-RIC8A monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-RIC8A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501055, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RIC8A (RC211359, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RIC8A.
Anti-RIC8A mouse monoclonal antibody (TA501055) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RIC8A (RC211359).
Immunofluorescent staining of HepG2 cells using anti-RIC8A mouse monoclonal antibody (TA501055).