Stem Cell Synergy Solution - Differentiate

AMSBIO now offers MatriMix Hydrogel and Extragel Matrix, versatile 3D culture substrates for stem cell culture, enabling multi-organ organoid formation and drug screening more representative of in vivo testing. Our 3D cell culture matrices mimic the natural ECM, providing a more physiologically relevant environment for stem cell differentiation, leading to improved functionality and maturity of differentiated cells. 

 Extragel

• Like-for-like replacement for Matrigel™, Geltrex™, and Cultrex™ BME 
• Composed mainly of laminin, collagen IV, heparan sulfate proteoglycans, and growth factors such as EGF, PDGF, NGF, FGF-2, TGF-β and IGF 
• Stringent quality control
• High lot-to-lot consistency 

StemFit® for Differentiation is a revolutionary chemically-defined and animal component-free supplement for differentiating ESCs and iPSCs into various lineages. It is compatible with a range different induction factors and cytokines, and can be combined with StemFit® culture media and iMatrix Recombinant Laminin E8 Fragments, including iMatrix-511 for a streamlined workflow.

• Highly consistent cell differentiation.
• Animal-origin free composition minimizes risk of immunogenic contamination.
• Promotes spontaneous differentiation of PSCs via embryoid body formation.
• Supports differentiation along ectoderm, mesoderm, or endoderm lineages. 
• Cost-effective, supplied as 5X concentrate.

iMatrix Recombinant Laminin E8 Fragments, offered by AMSBIO, are a type of extracellular matrix protein that plays a crucial role in cell adhesion, and can be used in cell culture to efficiently induce differentiation of pluripotent stem cells into various lineages. With a range of isoforms available, iMatrix Recombinant Laminin E8 Fragments can provide you with a versatile tool for directing stem cell fate and investigating differentiation processes. 

• Highly purified and refined laminin E8 fragments ensuring consistent and reliable results 
• Comprehensive range of isoforms, for differentiation into an array of lineages 
• Use in combination with StemFit Purotein® and StemFit® Culture Media for the ultimate differentiation solution

Our StemFit® Purotein Recombinant Proteins are a range of easy-to-use animal origin-free growth factors and cytokines. These enable precise differentiation of ESCs and iPSCs into a variety of lineages including liver, pancreas, kidney, heart, blood and immune cells. 

• Ready-to-use frozen formulation, no reconstitution necessary 
• High lot-to-lot consistency 
• High purity and high performance
• Available in research and clinical grade formats, and PMDA approved for cell therapy 

Our differentiation kits streamline your approach to generating mature specialised cell types from iPSCs. Downstream applications include disease modelling and drug screening approaches.

We have kits for generating multiple different cell types including:

• Skeletal muscle
• Neurons - GABAergic, Cholinergic, Dopaminergic

StemFit® for T-Cell Differentiation or StemFit® T-Diff is a chemically-defined animal-origin free supplement which enables researchers to differentiate pluripotent stem cells into T cells with consistently high differentiation performance. StemFit® for T-Cell Differentiation provides a controlled and reproducible approach to T cell generation, with a streamlined workflow. 

• Animal origin-free, minimizing the risk of viral and immunogenic contamination. 
• Chemically defined composition minimizes lot-to-lot variation and enables consistent differentiation performance 
• High percentage yield of T-cells
• Generates T-cells with high genetic stability 

Small molecules are cell permeable, organic compounds with low molecular weights, that can be used as tools to manipulate cell fates via the targeting of signalling pathways. This makes small molecules extremely useful in stem cell and regenerative medicine research. Our diverse range of small molecules, including all-trans retinoic acid, dorsomorphin, and doxycycline hyclate, promote differentiation across a variety of lineages.

Explore our range of small molecules, and advance your life science research today!

• Scientists at the University of Tokyo and Yokohama City University have developed an animal-derived product-free protocol for mass production of mini-livers, with the potential to transform liver disease treatment.
• Ajinomoto collaborated Professor Hideki Taniguchi at The University of Tokyo’s Department of Regenerative Medicine to create StemFit® for Differentiation, a chemically-defined, animal-origin free media (CD-AOF), enabling the development of an entirely animal-free protocol for iPSC-derived liver buds which also utilises iMatrix-511 Recombinant Laminin E8 Fragments, StemFit® Basic03, and StemFit Purotein® Activin A.
• Dr. Satoshi Okamoto and his team at Yokohama City University successfully optimised this protocol, achieving larger-scale stable production of iPSC-LBs for clinical applications.
• The CD-AOF approach demonstrated comparable efficiency to conventional methods, showing improved albumin secretion and higher expression of mature hepatic markers, opening new avenues for liver disease treatment.