Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503749)
HEK293T cells transfected with either RC217969 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RBBP7 antibody (TA503749), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RBBP7 (RC217969, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RBBP7. Positive lysates LY419038 (100ug) and LC419038 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-RBBP7 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503749)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RBBP7 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT, Cat# LC810HELA) and RBBP7-Knockout Hela cells (KO, Cat# LC810200) were separated by SDS-PAGE and immunoblotted with anti-RBBP7 monoclonal antibody TA503749. Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control (1:500).