Anti-RALBP1 mouse monoclonal antibody (TA500890) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1 (RC201524).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (RC201524, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RALBP1. Positive lysates LY402031 (100ug) and LC402031 (20ug) can be purchased separately from OriGene.
Immunoprecipitation (IP) of RALBP1 by using TrueMab monoclonal anti-RALBP1 antibodies (Negative control: IP without adding anti-RALBP1 antibody.). For each experiment, 500ul of DDK tagged RALBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-RALBP1 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either pCMV6-ENTRY RALBP1 (RC201524) (Red) or empty vector control plasmid (Blue) were immunostained with anti-RALBP1 mouse monoclonal (TA500890), and then analyzed by flow cytometry.