HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY RALBP1 (RC201524, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-RALBP
Immunoprecipitation (IP) of RALBP1 by using TrueMab monoclonal anti-RALBP1 antibodies (Negative control: IP without adding anti-RALBP1 antibody.). For each experiment, 500ul of DDK tagged RALBP1 overexpression lysates (at 1:5 dilution with HEK293T lysate)
HEK293T cells transfected with either RC201524 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-RALBP1 antibody (TA500908), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-RALBP1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Anti-RALBP1 mouse monoclonal antibody (TA500908) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY RALBP1 (RC201524).