HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY LIN28A (RC203397, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-LIN28. Positive lysates LY411135 (100ug) and LC411135 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
ELISA: LIN28 Antibody
Immunofluorescent staining of human cell line U-2 OS shows positivity in nucleus but not nucleoli.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.