HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD33 (RC207023, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD33. Positive lysates LY400667 (100ug) and LC400667 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of human bone marrow shows strong cytoplasmic positivity in bone marrow poietic cells.This validation was performed by Protein Atlas and the presentation of data is for informational purposes only.
Lane 1: Marker [kDa] 219, 111, 83, 48, 32, 26, 17; Lane 2: RT-4; Lane 3: U-251 MG; Lane 4: Human Plasma; Lane 5: Liver; Lane 6: TonsilThis validation was performed by Protein Atlas and the presentation of data is for informational purposes only.