HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PKLR (RC206455, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PKLR. Positive lysates LY400113 (100ug) and LC400113 (20ug) can be purchased separately from OriGene.
Anti-PKLR mouse monoclonal antibody (TA501416) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PKLR (RC206455).
HEK293T cells transfected with either RC206455 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PKLR antibody (TA501416), and then analyzed by flow cytometry.