Anti-PYCRL mouse monoclonal antibody (TA502034) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PYCRL (RC203382).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PYCRL (RC229141, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PYCRL. Positive lysates LY411510 (100ug) and LC411510 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PYCRL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502034)
HEK293T cells transfected with either RC203382 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PYCRL antibody (TA502034), and then analyzed by flow cytometry.