Advantages
・Full length membrane proteins with high purity
・High solubility in aqueous solutions
・High stability, lyophilized powder stable at room temperature
・Proteins are in a native membrane environment and remain biologically active
・No detergent, useful for cell-based assays
・No MSP backbone proteins
Applications
・ELISA
・SPR affinity analysis
・Phage display screening
・Immunization
・Cell based assays
・Protein crystal structure analysis
Advantages of Nanodiscs for Membrane Proteins
Membrane Proteins (MPs) account for more than 60% of all FDA-approved drug targets and 90% of antibody-based drug targets. To prepare functional multi-pass transmembrane protein, various cellular and cell-free expression systems have been employed. Besides Nanodisc, there are several solutions for multi-pass transmembrane protein preparation, including detergent, peptide made up of N-terminus or extracellular loops, membrane fraction, whole cell and VLP.
Detergent is widely used to produce multi-pass transmembrane proteins. However, one of the biggest downsides of using detergent is proteins need to be maintained in detergent-containing solutions, and detergent cannot be completely removed. This can be problematic for downstream applications, as the presence of detergent may interfere with certain experiments or affect the protein structure.
An alternative method to obtain proteins with good solubility and correct folding is to use peptides derived from the N-terminus or extracellular loops. However, the products may not resemble the structural features and conformation of the original extracellular loops of multi-pass transmembrane proteins in the native membrane.
Another option is using membrane fractions, whole cells or VLPs to maintain natural structure of multi-pass transmembrane proteins, but immunization with these products will result in high levels of nonspecific antibody background.
Comparing with these traditional solutions, Nanodisc platform offers a more advanced solution. It enables the production of full-length MPs with high purity and excellent solubility in aqueous solutions. Proteins incorporated into Nanodiscs are in native membrane environment, ensuring they remain biologically active. There is no detergent in these products, making them suitable to use in cell- based assays and other experiments.
Our Products
We have a wide variety of Nanodiscs to suit your needs based on your protein requirement. Please use the catalog results page and search by your protein.
All our nanodiscs come in 10μg, 50μg and 100μg for your convenience.
Validation Data
