Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-HPGD mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502450)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HPGD (RC204160, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HPGD. Positive lysates LY400305 (100ug) and LC400305 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC204160 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-HPGD antibody (TA502450), and then analyzed by flow cytometry.