HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRL (RC205627, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRL. Positive lysates LY424443 (100ug) and LC424443 (20ug) can be purchased separately from OriGene.
Anti-PRL mouse monoclonal antibody (TA500718) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRL (RC205627).
Flow cytometric Analysis of Jurkat cells, using anti-PRL antibody (TA500718), (Red), compared to a nonspecific negative control antibody, (Blue).
Immunofluorescent staining of HepG2 cells using anti-PRL mouse monoclonal antibody (TA500718).