Proper antibody validation requires the use of both positive and negative controls. Negative controls are essential to ensure the absence of nonspecific binding, the best ones being cell lines known not to contain your protein of interest. Cutting edge CRISPR/Cas9 gene-editing technology has been used to create knockout cell lines that achieve exactly this.
AMSBIO offers a range of knockout cell lysates developed using CRISPR and derived from commonly used cell lines. Our knockout lysates serve as ideal negative controls for western blots, and ultimately allow you to have confidence in the quality and specificity of your chosen antibody.