Immunohistochemistry (Paraffin-embedded sections) - Prion protein PrP antibody [EP1802Y]; Immunohistochemical analysis of brain glioma using TA300958 at a dilution of 1/100-1/250.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prion protein PrP antibody [EP1802Y]; Immunohistochemical analysis of Prion-infected mouse brain tissue, staining Prion protein PrP with TA300958. Antigen retrieval was performed by heat mediation in a citrate buffer (pH 6) before incubating with primary antibody (1/7000) overnight at 4Â°C. Staining was detected using DAB.
Figure from citation: Immunohistochemistry of PRNP protein level by using anti-PRNP antibody in mouse brain tissue sections. Prnp-positive signals were stained brown. +/+: wt mice; -/-: non-treated MPS IIIB mice; AAV9: rAAV9-treated MPS IIIB mice; #1: mouse with low Prnp IHC intensity; #2: mouse with high Prnp IHC intensity; DG: dentate gyrus of hippocampus. PL: polymoph layer of DG. Scale bar: 50 um. View Citation
Western blot ; Anti-Prion protein PrP antibody [EP1802Y] at 1/5000 dilution + Mouse Prion protein PrP full length protein at 0.01 ug.Secondary.Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution.developed using the ECL technique.Performed under reducing conditions.Exposure time : 10 seconds
Flow Cytometry-Anti-Prion protein PrP antibody (TA300958); Overlay histogram showing SH-SY5Y cells stained with TA300958 (red line). The secondary antibody used was DyLight 488 goat anti-rabbit IgG (H+L) at 1:500. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1ug/1x10^6 cells) used under the same conditions. This antibody gave a positive signal in SH-SY5Y cells under the same conditions.