HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (RC200009, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1. Positive lysates LY402509 (100ug) and LC402509 (20ug) can be purchased separately from OriGene.
Flow cytometric analysis of Hela cells, using anti-PPME1 antibody (TA500823), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).
Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500823, Dilution 1:50)
Anti-PPME1 mouse monoclonal antibody (TA500823) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1 (RC200009).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPME1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Flow cytometric analysis of Jurkat cells, using anti-PPME1 antibody (TA500823), (Red) compared to a nonspecific negative control antibody (TA50011) (Blue).