Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500821, Dilution 1:50)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPME1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-PPME1mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500821, Dilution 1:50)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPME1 (RC200009, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPME1. Positive lysates LY402509 (100ug) and LC402509 (20ug) can be purchased separately from OriGene.
Anti-PPME1 mouse monoclonal antibody (TA500821) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPME1 (RC200009).