HEK293T cells transfected with either RC212918 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PPM1B antibody (TA502865), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPM1B monoclonal antibody.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT, Cat# LC810293T) and PPM1B-Knockout 293T cells (KO, Cat# LC811002) were separated by SDS-PAGE and immunoblotted with anti-PPM1B monoclonal antibody TA502865, (1:500). Then the blotted membrane was stripped and reprobed with anti-HSP90AB1 antibody (TA500494) as a loading control.
Flow cytometric Analysis of Hela cells, using anti-PPM1B antibody (TA502865), (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PPM1B antibody (TA502865), (Red), compared to a nonspecific negative control antibody, (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPM1B (RC212918, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPM1B. Positive lysates LY419156 (100ug) and LC419156 (20ug) can be purchased separately from OriGene.