HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PPARA (RC216176, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PPARA. Positive lysates LY401560 (100ug) and LC401560 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PPARA monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PPARA mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503545)
Anti-PPARA mouse monoclonal antibody (TA503545) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PPARA (RC216176).