HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POLR2J2 (RC224755, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POLR2J2. Positive lysates LY409843 (100ug) and LC409843 (20ug) can be purchased separately from OriGene.
Anti-POLR2J2 mouse monoclonal antibody (TA502454) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY POLR2J2 (RC224755).
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-POLR2J2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502454)
HEK293T cells transfected with either RC224755 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-POLR2J2 antibody (TA502454), and then analyzed by flow cytometry.