HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PNMT (RC206586, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PNMT. Positive lysates LY400946 (100ug) and LC400946 (20ug) can be purchased separately from OriGene.
Anti-PNMT mouse monoclonal antibody (TA502825) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PNMT (RC206586 ).
HEK293T cells transfected with either RC206586 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PNMT antibody (TA502825), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-PNMT antibody (TA502825), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).