HEK293T cells transfected with either RC220376 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKAR2A antibody (TA501195), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR2A (RC220376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR2A. Positive lysates LY401337 (100ug) and LC401337 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PRKAR2A monoclonal antibody.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-PRKAR2A monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501195, Dilution 1:50)
Anti-PRKAR2A mouse monoclonal antibody (TA501195) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR2A (RC220376 ).