HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR2A (RC220376, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR2A. Positive lysates LY401337 (100ug) and LC401337 (20ug) can be purchased separately from OriGene.
Equivalent amounts of cell lysates (10 ug per lane) of wild-type HeLa cells (WT, Cat# LC810HELA) and PRKAR2A-Knockout HeLa cells (KO, Cat# LC812853) were separated by SDS-PAGE and immunoblotted with anti-PRKAR2A monoclonal antibody TA501104 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Immunohistochemical staining of paraffin-embedded prostate tissue within the normal limits using anti-PRKAR2A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501104, Dilution 1:50)
Anti-PRKAR2A mouse monoclonal antibody (TA501104) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR2A (RC220376).
HEK293T cells transfected with either RC220376 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PRKAR2A antibody (TA501104), and then analyzed by flow cytometry.