HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIM2 (RC201933, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIM2. Positive lysates LY416355 (100ug) and LC416355 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501062)
Immunoprecipitation (IP) of PIM2 by using TrueMab monoclonal anti-PIM2 antibodies (Negative control: IP without adding anti-PIM2 antibody.). For each experiment, 500ul of DDK tagged PIM2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-PIM2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.
HEK293T cells transfected with either RC201933 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIM2 antibody (TA501062), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-PIM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501062)
Anti-PIM2 mouse monoclonal antibody (TA501062) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIM2 (RC201933).