IF image of Hela cell stained with PI3KCD Antibody (N-term) (Cat#TA325015).Hela cells were incubated with PI3KCD primary antibody (1:25, 1 h at 37Â°C). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7 units/ml).PI3KCD immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis of anti-PI3KCD Antibody (N-term) Pab (TA325015) in 293 cell line lysates. PI3KCD Antibody (N-term) (arrow) was detected using the purified Pab.
Formalin-fixed and paraffin-embedded human breast carcinoma reacted with PI3KCD Antibody (N-term) (Cat.#TA325015), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.