HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PIK3AP1 (RC214125, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PIK3AP1. Positive lysates LY403461 (100ug) and LC403461 (20ug) can be purchased separately from OriGene.
Anti-PIK3AP1 mouse monoclonal antibody (TA501760) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PIK3AP1 (RC214125).
Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PIK3AP1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501760, Dilution 1:50)
HEK293T cells transfected with either RC214125 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PIK3AP1 antibody (TA501760), and then analyzed by flow cytometry.