Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PGAM2 monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PGAM2 (RC200701, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PGAM2. Positive lysates LY424823 (100ug) and LC424823 (20ug) can be purchased separately from OriGene.
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-PGAM2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503425)
Anti-PGAM2 mouse monoclonal antibody (TA503425) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PGAM2 (RC200701).
Immunofluorescent staining of HeLa cells using anti-PGAM2 mouse monoclonal antibody (TA503425).
HEK293T cells transfected with either RC200701 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PGAM2 antibody (TA503425), and then analyzed by flow cytometry.