IF image of Hela cell stained with PRDX2 Antibody (C-term) (Cat#TA324756).Hela cells were incubated with PRDX2 primary antibody (1:25, 1 h at 37Â°C). For secondary antibody, Alexa FluorÂ® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa FluorÂ® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue).PRDX2 immunoreactivity is localized to Cytoplasm significantly.
Western blot analysis of lysates from mouse heart and rat heart tissue lysate (from left to right), using PRDX2 Antibody (C-term) (Cat. #TA324756). TA324756 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Western blot analysis of PRDX2 Antibody (C-term) (Cat. #TA324756) in MDA-MB231, 293 cell line and mouse brain tissue lysates (35ug/lane).PRDX2 (arrow) was detected using the purified Pab. (2ug/ml)
Formalin-fixed and paraffin-embedded human brain tissue reacted with PRDX2 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.