FACS analysis with primary human dermal microvascular (HDMVEC) and umbilical vein (HUVEC) endothelial cells. The lymphatic endothelial cell marker Podoplanin is not expressed in the blood endothelial cells.
Western blot analysis of Podoplanin expression in Human lymphatic endothelial cells (LEC) and HUVECs. Total lysate of both cell types were subjected to SDS-PAGE and subsequent Western analysis with the Podoplanin Antibody. The antibody recognizes a protein of about 36 kDa in total lysate from LECs but not from HUVEC.
FACS analysis with Human primary lymphatic endothelial cells (HDLEC). As secondary antibody anti-Mouse IgG-PE was used.
Immunofluorescence staining (green) of Podoplanin in primary human dermal lymphatic endothelial cells (HDLEC) with anti-Human Podoplanin Antibody V (0.5 ug/ml) and counter staining of nuclei with Dapi. As secondary antibody Goat anti-Mouse ALEXA Fluor 488 was used 1/400.