HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDLIM2 (RC210022, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDLIM2. Positive lysates LY411957 (100ug) and LC411957 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDLIM2 monoclonal antibody.
Flow cytometric Analysis of Jurkat cells, using anti-PDLIM2 antibody (TA502711), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells transfected with either RC210022 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDLIM2 antibody (TA502711), and then analyzed by flow cytometry.