Western blot analysis of extracts (10ug) from 9 Human tissue by using anti-PDF monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).
Flow cytometric Analysis of Hela cells, using anti-PDF antibody (TA502988), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PDF antibody (TA502988), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (RC205788, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDF. Positive lysates LY411712 (100ug) and LC411712 (20ug) can be purchased separately from OriGene.
Anti-PDF mouse monoclonal antibody (TA502988) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF (RC205788 ).
HEK293T cells transfected with either RC205788 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDF antibody (TA502988), and then analyzed by flow cytometry.