Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDF monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Anti-PDF mouse monoclonal antibody (TA502989) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDF (RC205788 ).
HEK293T cells transfected with either RC205788 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDF antibody (TA502989), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDF (RC205788, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDF. Positive lysates LY411712 (100ug) and LC411712 (20ug) can be purchased separately from OriGene.
Flow cytometric Analysis of Hela cells, using anti-PDF antibody (TA502989), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).