PDE7A-HEK293 Recombinant Cell Line (Rat)

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AMS.60408
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Recombinant HEK293 cell line expressing rat PDE7A (phosphodiesterase 7A/ accession number NM_031080). N-terminal FLAG-tagged rat PDE7A has been stably expressed in a human embryonic kidney (HEK293) cell line and its expression was confirmed by Western blotting. The regulation of intracellular level of cAMP by rat PDE7A in rat PDE7A stably-expressed HEK293 cells was characterized by a cell-based reporter assay using pCRE-luc reporter vector. pCRE-luc contains a luciferase gene that is under the control of the cAMP response element (CRE). When cells transiently transfected with pCRE-luc reporter were activated by forskolin/ the level of cAMP was upregulated in parental HEK293 cells inducing the expression of luciferase reporter/ whereas rat PDE7A-HEK293 cells showed reduction in the level of cAMP/ resulting in lowered levels of luciferase expression. Inhibition of PDE7A activity by BRL 50481/ a PDE7A inhibitor/ restored the cAMP level/ resulting in higher luciferase activity.
More Information
SKU AMS.60408
Size 2 vials
Species Rat
Cell Line HEK293
Tissue Type Kidney
Application Useful to monitor cAMP and PDE7A signaling pathway activity and to screen activators or inhibitors of PDE7A.
Accession No NM_031080
Gene id NM_031080
Gene synonyms PDE7A, phosphodiesterase 7A
Storage Store in liquid nitrogen immediately upon receipt.
Shipping Temp -80C (dry ice)
Supplier Name BPS
For more details visit our Phosphodiesterases page >