HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE4B (RC211956, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE4B. Positive lysates LY400919 (100ug) and LC400919 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC211956 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE4B antibody (TA503532), and then analyzed by flow cytometry.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDE4B monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503532)
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-PDE4B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503532)