HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE2A (RC207219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE2A. Positive lysates LY419215 (100ug) and LC419215 (20ug) can be purchased separately from OriGene.
Anti-PDE2A mouse monoclonal antibody (TA502319) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE2A (RC207219).
HEK293T cells transfected with either RC207219 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE2A antibody (TA502319), and then analyzed by flow cytometry.