HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE2A (RC207219, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE2A. Positive lysates LY419215 (100ug) and LC419215 (20ug) can be purchased separately from OriGene.
HEK293T cells transfected with either RC207219 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PDE2A antibody (TA502683), and then analyzed by flow cytometry.
Flow cytometric Analysis of Hela cells, using anti-PDE2A antibody (TA502683), (Red), compared to a nonspecific negative control antibody, (Blue).
Flow cytometric Analysis of Jurkat cells, using anti-PDE2A antibody (TA502683), (Red), compared to a nonspecific negative control antibody, (Blue).
Anti-PDE2A mouse monoclonal antibody (TA502683) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PDE2A (RC207219).