Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PDE10A monoclonal antibody (1:200).
Immunohistochemical staining of paraffin-embedded endometrium tissue within the normal limits using anti-PDE10A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA500635, Dilution 1:50)
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-PDE10A monoclonal antibody.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PDE10A (RC211181, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PDE10A. Positive lysates LY416496 (100ug) and LC416496 (20ug) can be purchased separately from OriGene.