Flow cytometric Analysis of stable expression CD274 cells using anti-CD274 antibody (TA809809) (Red) compared to a nonspecific negative control antibody (Blue). The left is 293T as negative control (1:100).
PD-L1 ELISA with 13G7 Capture (TA809809) and 9E12 Detection (TA808771) Antibodies. Substrate used: Recombinant Human PD-L1 (TP700201)
Flow cytometric Analysis of HCC78 cells, using anti-PDL1 antibody (TA809809), (Red), compared to isotype control, (green), and negative control (PBS), (Blue) (1:100).
Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right)/untreated (Left) using anti-PDL1 antibody (TA809809) (1:100).
Immunocytochemistry staining of CD274 stable expression cells using anti-CD274 mouse monoclonal antibody (TA507087) (Left). The right is negative control (1:5000).
Flow cytometric analysis of living PBMCs treated with 10ug/ml PHA for 72h (Right) using anti-PDL1 antibody (TA809809). Cells incubated with a non-specific antibody (Left) were used as isotype control (1:100).
Detection of PDL1 neutralizing antibody using MACS column. GFP+/PDL1+ 293T cells (co-transfected with PDL1 and GFP plasmid (RC213071, PS10010) were incubated with either PDL1 antibody TA809809 (red), non-specific antibody (green), isotype control (blue) or PBS (black) and then mixed with PD1+ 293T cells (RC210364) linked with magnetic-beads. The mixed cells were pulled down using MACS column (Miltenyi Biotec) and analysed by Flow Cytometry. GFP+/PDL1+ cells would not be collected if PD1/PDL1 interaction is neutralized by the tested antibody (1:50).