Anti-PAICS mouse monoclonal antibody (TA501469) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PAICS (RC215225).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAICS (RC215225, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAICS. Positive lysates LY416636 (100ug) and LC416636 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from 10 Human tissue by using anti-PAICS monoclonal antibody at 1:500 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon;10: spleen).
HEK293T cells transfected with either RC215225 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PAICS antibody (TA501469), and then analyzed by flow cytometry.