HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PAICS (RC215225, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PAICS. Positive lysates LY416636 (100ug) and LC416636 (20ug) can be purchased separately from OriGene.
Western blot analysis of extracts (10ug) from 5 different cell lines by using anti-PAICS monoclonal antibody at 1:200 dilution.
HEK293T cells transfected with either RC215225 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-PAICS antibody (TA501470), and then analyzed by flow cytometry.
Flow cytometric Analysis of Jurkat cells, using anti-PAICS antibody (TA501470), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Anti-PAICS mouse monoclonal antibody (TA501470) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PAICS (RC215225).