Anti-TP53 mouse monoclonal antibody (TA502851) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TP53 (RC200003).
Flow cytometric Analysis of Hela cells, using anti-TP53 antibody (TA502851), (Red), compared to a nonspecific negative control antibody, (Blue).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TP53 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-TP53 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA502851)
HEK293T cells transfected with either RC200003 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TP53 antibody (TA502851), and then analyzed by flow cytometry.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TP53 (RC200003, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TP53. Positive lysates LY400186 (100ug) and LC400186 (20ug) can be purchased separately from OriGene.
Flow cytometric Analysis of Jurkat cells, using anti-TP53 antibody (TA502851), (Red), compared to a nonspecific negative control antibody, (Blue).