HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ODC1 (RC206858, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ODC1. Positive lysates LY400909 (100ug) and LC400909 (20ug) can be purchased separately from OriGene.
Flow cytometric Analysis of Jurkat cells, using anti-ODC1 antibody (TA501286), (Red), compared to a nonspecific negative control antibody (TA50011), (Blue).
Anti-ODC1 mouse monoclonal antibody (TA501286) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY ODC1 (RC206858).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-ODC1 monoclonal antibody.
HEK293T cells transfected with either RC206858 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ODC1 antibody (TA501286), and then analyzed by flow cytometry.