ONE-Stepâ„¢ Luciferase Assay System

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The One-Step Luciferase Assay System is designed to be used for high-throughput, sensitive quantitation of firefly luciferase activity in mammalian cell culture. The reagent consists of two components, a Luciferase Reagent Buffer (Component A) and Luciferase Reagent Substrate (Component B). Component A and Component B are combined to form a working solution that contains all the necessary components for cell lysis and luciferase quantitation. This assay system has several features:
Sensitive - highly sensitive detection of firefly luciferase activity.
Stable - the signal output is stable for more than two hours, providing flexibility with regard to incubation time
Convenient - simple one-step, homogeneous protocol.
High-throughput - one-step homogeneous protocol minimizes handling steps to support high-throughput screening applications
Compatibility - works well with a variety of common media containing 0-10% serum and phenol red.
Instrumentation - does not require a luminometer with injectors.
More Information
SKU AMS.60690-1
Size 10 ml
Application Monitor firefly (Photinus pyralis) luciferase activity in cultured mammalian cells. High-throughput screening using luciferase reporter cell lines
Gene synonyms Luciferin, Firefly luciferase, luciferase buffer
Storage At least 6 months when stored as directed. Upon first thaw, store in aliquots at -20C. Thereagent may be subjected to several freeze/thaw cycles with no effect on functionality, but it isrecommended that freeze/thaw cycles be avoided whenever possible.
Shipping Temp -20C (gel packs or dry ice)
Supplier Name BPS
Research Areas Immunology
For more details visit our Luciferase Assay Systems page >