Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-OBFC1 monoclonal antibody at 1:200 dilution.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY OBFC1 (RC200778, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OBFC1. Positive lysates LY410967 (100ug) and LC410967 (20ug) can be purchased separately from OriGene.
Anti-OBFC1 mouse monoclonal antibody (TA503240) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OBFC1 (RC200778).
HEK293T cells transfected with either RC200778 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-OBFC1 antibody (TA503240), and then analyzed by flow cytometry.