HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NXNL2 (RC205425, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NXNL2. Positive lysates LY408006 (100ug) and LC408006 (20ug) can be purchased separately from OriGene.
Anti-NXNL2 mouse monoclonal antibody (TA501215) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NXNL2 (RC205425 ).
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NXNL2 monoclonal antibody.
Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-NXNL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501215)
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-NXNL2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA501215)