Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-NDEL1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) (1:200).
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503352)
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NDEL1 (RC212323, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NDEL1. Positive lysates LY422448 (100ug) and LC422448 (20ug) can be purchased separately from OriGene.
Anti-NDEL1 mouse monoclonal antibody (TA503352) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY NDEL1 (RC212323).
HEK293T cells transfected with either RC212323 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-NDEL1 antibody (TA503352), and then analyzed by flow cytometry.
Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-NDEL1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100??C for 10min, TA503352)